Jan van den Bossche

About

Jan Van den Bossche is now Associate Professor at the Department of Molecular Cell Biology and Immunology at Amsterdam UMC. He leads the Translational Macrophage Immunometabolism group IMMUNOMETLAB. His young and enthusiastic team specializes in the immune/metabolic profiling and targeting of macrophages and other immune cells, particularly in the context of cancer and cardiometabolic

Research Line

Jan Van den Bossche’s lab performs pioneering research on macrophage immunometabolism. The overall aim is to explain how metabolic reprogramming regulates macrophage subsets in different settings, focusing on cancer and cardiovascular disease. By unravelling key questions in macrophage immunometabolism, the ultimate goal is to demonstrate whether and how targeting macrophage metabolism can be used for future therapy. Current research topics include immunometabolites (succinate, itaconate and new-found ones), metabolic immune-cancer interactions, metabolic enzymes (including ACLY; ATP citrate lyase).

Over the past decade, Jan’s team established an immunometabolic profiling platform allowing the phenotypic, functional and metabolic characterization of macrophage and other immune subsets in a fast and cost-effective way

In-house techniques include extracellular flux analysis (Seahorse), metabolomics, Cytation multi-mode imaging plate reader, transcriptomics, and cytometry- and imaging-based single-cell metabolic profiling.

Our distinct research lines investigate how metabolic enzymes like ATP Citrate Lyase and (immuno)metabolites like succinate and itaconate control macrophage responses and disease progression.

Key publications

Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques
Baardman, J., Verberk, S. G. S., van der Velden, S., Gijbels, M. J. J., van Roomen, C. P. P. A., Sluimer, J. C., Broos, J. Y., Griffith, G. R., Prange, K. H. M., van Weeghel, M., Lakbir, S., Molenaar, D., Meinster, E., Neele, A. E., Kooij, G., de Vries, H. E., Lutgens, E., Wellen, K. E., de Winther, M. P. J. & Van den Bossche, J., 1 Dec 2020, In: Nature communications. 11, 1, 6296.
Immunometabolism in the Single-Cell Era
Artyomov, M. N. & Van den Bossche, J., 3 Nov 2020, In: Cell metabolism. 32, 5, p. 710-725 16 p.
Single-cell metabolic profiling of human cytotoxic T cells
Hartmann, F. J., Mrdjen, D., McCaffrey, E., Glass, D. R., Greenwald, N. F., Bharadwaj, A., Khair, Z., Verberk, S. G. S., Baranski, A., Baskar, R., Graf, W., Van Valen, D., Van den Bossche, J., Angelo, M. & Bendall, S. C., Feb 2021, In: Nature biotechnology. 39, 2, p. 186-197 12 p.